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    "Frames": 300,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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    "Interval_ms": 10000,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "FileName": "img_000000001_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 1,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "COM4-Parity": "None",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Width": 1004,
  "WaitInterval": null,
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000002_ETGFP_000.tif",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 2,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:30:33 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 22200,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000002_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-3-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 3,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:30:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Frames": 300,
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  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
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  "Camera": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000003_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 4,
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  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 4,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:30:53 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 41519,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 4,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000004_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-4-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 4,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 4,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:30:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "5f1c28f1-a365-48b4-b305-e4604ac5f987",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 41644,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 4,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000004_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-4-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 4,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 5,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:30:53 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 42134,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 4,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000004_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-5-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 5,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 5,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:31:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "8deb55ea-a0ae-4fd6-adb9-b12fef2382f4",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 51571,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 5,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000005_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-5-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 5,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 5,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:31:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "c970fda5-cd58-41de-aaa1-1cd5c1e5b8cb",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 51692,
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Time": "2015-08-26 18:31:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000006_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "SliceIndex": 0,
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  "FrameIndex": 6,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:31:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "COM3-Handshaking": "Off",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 62099,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "COM3-DataBits": "8",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000006_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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},
"FrameKey-7-0-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 7,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
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  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000007_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
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  "Andor-FanMode": "Full",
  "FrameIndex": 7,
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
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  "Camera": "",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:31:23 -0700",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000007_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 7,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "2",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter Opening Time": "20",
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  "Andor-TimeOut": "10000",
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  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Width": 1004,
  "WaitInterval": null,
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000008_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 9,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:31:43 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000009_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-9-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 9,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 9,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:31:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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      1002
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    "Comment": "",
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    "GridColumn": 0,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000009_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "FrameIndex": 9,
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:31:43 -0700",
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  "XYStage-Port": "COM3",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Exposure": "100.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000009_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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"FrameKey-10-0-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 10,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "Core-XYStage": "XYStage",
  "AxisPositions": null,
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "1",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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      {
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
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  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:31:53 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "Slice": 0,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "ElapsedTime-ms": 101621,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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      {
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000010_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 10,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
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  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 11,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:31:53 -0700",
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  "COM1-Parity": "None",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "c9a48430-59da-484a-a5e2-6a5e2486afd4",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 102093,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "Andor-Shutter Opening Time": "20",
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        "GridColumnIndex": 0,
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      {
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
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  "COM3-DataBits": "8",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000010_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-11-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
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  "FrameIndex": 11,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIFilterBlock1-State": "1",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000011_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 11,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:32:03 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 111608,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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      {
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000011_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-11-2-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 11,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:32:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "f4ed6002-27a0-4dbc-949a-4c894317b6fa",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 112079,
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 12,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 18:32:13 -0700",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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      0,
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000012_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 13,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:32:23 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "UUID": "e5ffb63b-b093-4161-b488-7639b0beed6e",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 131496,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000013_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-13-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 13,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:32:23 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000013_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 13,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 14,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:32:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "ede6e833-6c60-4517-b3a6-fbe8fea4d9b3",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 132132,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 13,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000013_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-14-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 14,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 14,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:32:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "ec002ab1-a140-401d-9905-73b18be142be",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 141492,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 15,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "138.19",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "1",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000015_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 15,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:32:43 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "938caaf1-75e1-4393-97ad-10114893573c",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 151607,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000015_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 15,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 16,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:32:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
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        "GridColumnIndex": 0,
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    "Frames": 300,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "IJType": 1,
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
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  "Andor-TransposeCorrection": "0",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:32:53 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "Slice": 0,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "PixelAspect": 1,
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000016_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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"FrameKey-17-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "COM1-Verbose": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 17,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "Core-XYStage": "XYStage",
  "AxisPositions": null,
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 171635,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000017_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
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  "FrameIndex": 17,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
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  "TIFilterBlock1-State": "1",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
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  "Shutter-A-Port": "COM1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000017_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 17,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:33:03 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "1215a491-047c-4217-b7bb-5f169a381eee",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 172222,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000017_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-18-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 18,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 18,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:33:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "bb1c0636-1562-492f-9ba4-03ffb016bb7b",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 181536,
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Core-Galvo": "",
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  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "Time": "2015-08-26 18:33:23 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000019_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 19,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:33:23 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "fe24deed-51ba-4007-9132-8507406b353f",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 191599,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "COM3-StopBits": "1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000019_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-19-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 19,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-TransposeCorrection": "0",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000019_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
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  "Andor-FanMode": "Full",
  "FrameIndex": 20,
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  "XYStage-BuildName": "STD_XY",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000020_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 20,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "273c9fbc-20e6-4cd1-8315-0c84dcd5cf96",
  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
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    "ComputerName": "vulcan_computer",
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "FileName": "img_000000021_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 22,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
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  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "SlicesFirst": false,
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "COM3-Parity": "None",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
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  "FileName": "img_000000022_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 22,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
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  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
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  "Andor-FanMode": "Full",
  "FrameIndex": 23,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 24,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:34:03 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "UUID": "1edbb578-976f-438b-a65f-037795ca6089",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 232124,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 23,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "ETGFP",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000023_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-24-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 24,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 24,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:34:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "ae5eadc1-29dd-4d8e-bd1d-8dfe7325c009",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 241483,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 24,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000024_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-24-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 24,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 24,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:34:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "d66d9b1e-5c93-4e8a-83da-e170f96e0acd",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
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  "FrameIndex": 26,
  "XYStage-Version": "Version: USB-9.2d ",
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  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
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  "Camera": "",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 26,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:34:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "4d6c62bc-d649-4aaa-92a9-6601e2f91dc0",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 261658,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 26,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
          11463.9,
          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
      }
    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
    ],
    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
      0,
      0,
      1004,
      1002
    ],
    "ChNames": [
      "Phase (ETGFP)",
      "ETGFP",
      "TRITC"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
      -1,
      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000026_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-26-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 26,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 26,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:34:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "1e594441-0046-433e-acd6-422f4541f4dc",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 261773,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 26,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
      }
    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
    ],
    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
      0,
      0,
      1004,
      1002
    ],
    "ChNames": [
      "Phase (ETGFP)",
      "ETGFP",
      "TRITC"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
      -1,
      -13369600,
      -65536
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
    ],
    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000026_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-26-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 26,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 27,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:34:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "ce83c75c-d123-47dd-9b54-7a05018833a0",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 262243,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 26,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 28,
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  "COM1-AnswerTimeout": "500.0000",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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    "InitialPositionList": [
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
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    "ComputerName": "vulcan_computer",
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000028_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 28,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
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  "Andor-Shutter Closing Time": "20",
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  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:34:53 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 28,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000028_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 28,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 29,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:34:53 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "92ddeae1-b1ed-4ddf-b4c2-2ad9b3ccecb2",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 282123,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000028_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-29-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 29,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 29,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:35:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "XYStage-Port": "COM3",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "9425743b-e701-4285-9d83-014abf1a0e2f",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 291484,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
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    "Frames": 300,
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    "Frames": 300,
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Trigger": "Software",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 30,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
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  "NextFrame": 30,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:35:13 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 301488,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 30,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000030_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-30-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 30,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 30,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:35:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "25af1fc7-c453-4048-a402-d8e56a342d58",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 301605,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 30,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000030_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-30-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 30,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 31,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:35:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "eea00d1a-fcf4-4e25-9da3-8d6fa5b49253",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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    "Frames": 300,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "TimeFirst": false,
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000031_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
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  "FrameIndex": 31,
  "XYStage-Version": "Version: USB-9.2d ",
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000031_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 31,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 32,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:35:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "bfa5386d-21b3-4b6f-8dd3-2ba90af88932",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 312072,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 31,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000031_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-32-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 32,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 32,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:35:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "d564a1ec-00bc-4eb9-bf84-4d8d1b9e6eab",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 321487,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 32,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000032_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-32-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 32,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 32,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:35:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "847e69a5-ee3e-4334-b893-99d625aef3f7",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 321601,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 32,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000032_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-32-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 32,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 33,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:35:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "d5c793f6-431b-4749-af9a-8d6dc7078275",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 322075,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 32,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
      }
    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
    ],
    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
      "Phase (ETGFP)",
      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000032_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-33-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 33,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 33,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:35:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "d2429859-1f38-4248-9923-e35f255a0be6",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 331482,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 33,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
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    "Frames": 300,
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:35:43 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
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  "XPositionUm": 11577.1,
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  "Position": "Default",
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    "Frames": 300,
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  "IntensiLightShutter-Port": "COM4",
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    "Directory": "D:\\Rico Vulcan\\082615"
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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    "Interval_ms": 10000,
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  "Time": "2015-08-26 18:36:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000036_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 36,
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:36:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000036_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 37,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
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  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:36:23 -0700",
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  "COM1-Parity": "None",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000037_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 38,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 38,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:36:33 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 381653,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 38,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000038_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-38-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 38,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 38,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:36:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "ea099cb2-b024-4ce6-a39e-edbe97525454",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 381765,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 38,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000038_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 38,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:36:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000038_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 39,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:36:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 391501,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000039_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-39-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 39,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 39,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:36:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "f78103b6-c369-4e99-bab4-7e272b4de986",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 391615,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 40,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
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  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Time": "2015-08-26 18:36:53 -0700",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "1",
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  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000040_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 40,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:36:53 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "54f543cc-cfa7-4946-a66d-8b8d6df7d9e7",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 402103,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000040_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-41-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 41,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 41,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:37:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 42,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-Error-E(nm)": "400.0000",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 18:37:13 -0700",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000042_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 43,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:37:23 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 431502,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
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  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000043_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-43-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 43,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 43,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:37:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 44,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000044_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-44-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 44,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 44,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:37:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "f41f8401-73f5-48db-acb1-55273eebe45e",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 441766,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 44,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
      }
    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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      "ETGFP",
      "TRITC"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
      -1,
      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000044_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-44-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 44,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 45,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:37:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "a45ea193-f3d6-4f19-a49c-c6c2a3a55eaf",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 442235,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 44,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
      "Phase (ETGFP)",
      "ETGFP",
      "TRITC"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000044_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-45-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 45,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Time": "2015-08-26 18:37:43 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "1",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000045_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 45,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:37:43 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 451620,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000045_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-45-2-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 45,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 46,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:37:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "ff4ac68c-cf17-44e4-82d0-718cee5f46eb",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 452092,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
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  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000046_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 47,
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  "ROI": "0-0-1004-1002",
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  "Camera": "",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:38:03 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000047_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 47,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "NextFrame": 47,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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    "SlicesFirst": false,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000047_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 48,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "Time": "2015-08-26 18:38:23 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Width": 1004,
  "WaitInterval": null,
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000049_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Andor-FanMode": "Full",
  "FrameIndex": 49,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:38:23 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "BitDepth": 14,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 491642,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000049_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 49,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:38:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Time": "2015-08-26 18:30:13 -0700",
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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    "Interval_ms": 10000,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "FileName": "img_000000050_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 50,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "COM4-Parity": "None",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 51,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:38:33 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 502069,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 50,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000050_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-51-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 51,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 51,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:38:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "8dbb86f2-934d-4214-91eb-30852339a03a",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 511650,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000051_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-51-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 51,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
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  "Andor-PixelType": "16bit",
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  "XYStage-Error-E(nm)": "400.0000",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
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    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000051_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 51,
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
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  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:38:43 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "COM3-AnswerTimeout": "500.0000",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 512240,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000051_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-52-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 52,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:38:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "COM4-BaudRate": "9600",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "13c57bc1-d5fc-467c-8f6a-0ed31d237d07",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 521482,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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  "IntensiLightShutter-Port": "COM4",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Description": "Andor camera adapter",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Trigger": "Software",
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  "Position": "Default",
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  "Andor-FanMode": "Full",
  "FrameIndex": 53,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "COM4-Parity": "None",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:39:03 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000053_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-54-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 54,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 54,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:39:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "3a40545c-87e6-4621-b3af-e8c6c40afce3",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 541625,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 54,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000054_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-54-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 54,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 54,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:39:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "45c3057d-dd13-4ff7-9606-d8c936c66c28",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 541737,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 54,
  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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        "GridColumnIndex": 0,
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    "Date": "2015-08-26",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 55,
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Time": "2015-08-26 18:39:23 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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    "InitialPositionList": [
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "FileName": "img_000000055_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 56,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "COM1-Parity": "None",
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  "XYStage-Port": "COM3",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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    "Frames": 300,
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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    "Source": "Micro-Manager",
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    "TimeFirst": false,
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000057_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "FrameIndex": 57,
  "XYStage-Version": "Version: USB-9.2d ",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIFilterBlock1-State": "2",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "WaitInterval": null,
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000057_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 58,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:39:53 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 581643,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
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  "Binning": "1",
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  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000058_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-58-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 58,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 58,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:39:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "c6cfeece-3475-421d-9881-550a6b7021cc",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 581755,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
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  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
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  "Andor-FanMode": "Full",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000059_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 59,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "XYStage-Backlash-B": "0.0400",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:40:03 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "COM3-AnswerTimeout": "500.0000",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "faa171aa-c821-4b38-b710-a05706f3de92",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 592215,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000059_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-60-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 60,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "NextFrame": 60,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:40:13 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 601480,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 60,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000060_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-60-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 60,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 60,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:40:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "812a3eec-6403-49f2-9fc2-2106e609be15",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 601591,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 60,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000060_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-60-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 60,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 61,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:40:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "a80336de-a0dc-43ef-8ea9-d206b48889e8",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 602098,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 60,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-FanMode": "Full",
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  "TIFilterBlock1-Label": "3-TRITC",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-ChannelGroup": "Channel",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000061_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 62,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:40:33 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000062_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 62,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 62,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:40:33 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 63,
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "NextFrame": 63,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:40:43 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
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  "COM3-Handshaking": "Off",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 631641,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 63,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000063_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-63-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 63,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 64,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:40:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "de92f012-1b05-45ee-8b22-71adc8476c72",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 632107,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000063_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-64-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 64,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:40:53 -0700",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000064_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 64,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:40:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "3817bcb6-6840-4b18-9e91-f0e58a57deac",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 641592,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 64,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000064_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-64-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 64,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 65,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:40:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "5b7de884-d363-4908-acc7-792403caf268",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 642059,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Time": "2015-08-26 18:41:03 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000065_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 66,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:41:13 -0700",
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  "COM3-AnswerTimeout": "500.0000",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "ElapsedTime-ms": 661482,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000066_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 66,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 66,
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:41:13 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 66,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000066_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 66,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 67,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:41:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "f651d6c9-547c-48b7-b840-ad17b7b17e29",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 662060,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 66,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000066_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-67-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 67,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 67,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:41:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "5740c111-6763-405e-9b34-a62b41fa35e9",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 671481,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Width": 1004,
  "WaitInterval": null,
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000068_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 68,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:41:33 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 681759,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000068_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 68,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "TILightPath-Label": "2-Left100",
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  "NextFrame": 69,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:41:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 70,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:41:53 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "16cdabdd-6707-40a7-a756-5f519f7491e2",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 701593,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 70,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000070_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-70-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 70,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 71,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:41:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "bb4fa1f5-dce6-42f8-8618-6028c10a047d",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 702060,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 70,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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      "ETGFP",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000070_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-71-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 71,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 71,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:42:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "b235eac7-b2af-4fa1-ae90-4e532c9685b5",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 711479,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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    "PixelType": "GRAY16",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000072_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "FrameIndex": 72,
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:42:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000072_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 72,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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    "Frames": 300,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Trigger": "Software",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-FanMode": "Full",
  "FrameIndex": 73,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Shutter": "IntensiLightShutter",
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  "Andor-Trigger": "Software",
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-FanMode": "Full",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000074_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "FrameIndex": 74,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:42:33 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000074_TRITC_000.tif",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 75,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Time": "2015-08-26 18:30:13 -0700",
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    "PositionIndex": 1,
    "SlicesFirst": false,
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Trigger": "Software",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 75,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
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  "Camera": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
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    "TimeFirst": false,
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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    "Frames": 300,
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000076_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-77-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 77,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:43:03 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "3e92ac4a-07ae-476b-91fd-276901db5d84",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 771493,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000077_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-77-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 77,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 77,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:43:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "edd6a927-1c06-4c77-ae35-8469421a81fb",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 771606,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "FrameIndex": 78,
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "InitialPositionList": [
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
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    "ComputerName": "vulcan_computer",
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000078_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 79,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:43:23 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000079_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 79,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:43:23 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "784e4928-c7e1-43f1-812e-c92354722184",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 791758,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 79,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000079_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-79-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 79,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:43:23 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "74a91d66-b53e-49df-8270-8caa68387730",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 792225,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 80,
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 18:43:33 -0700",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000080_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 81,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:43:43 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 811501,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000081_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 81,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 81,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:43:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "TIFilterBlock1-State": "1",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000081_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 81,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:43:43 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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      {
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000081_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-82-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 82,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:43:53 -0700",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "ElapsedTime-ms": 821656,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "ChNames": [
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 83,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:44:03 -0700",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000083_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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"FrameKey-83-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 83,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 83,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:44:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "3fb51b2e-5d29-4185-b99b-c8cf26209f73",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 831593,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 83,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000083_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-83-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 83,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 84,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:44:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "084526d3-c385-46f7-b92e-d3467074f2a8",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000085_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 85,
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:44:23 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 852259,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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      {
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000085_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 86,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
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  "XYStage-JoyStick Fast Speed": "60",
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  "ElapsedTime-ms": 861652,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "PixelType": "GRAY16",
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    "SlicesFirst": false,
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    "Comment": "",
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    "GridColumn": 0,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
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  "Andor-Trigger": "Software",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:44:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Slice": 0,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "20.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000086_ETGFP_000.tif",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 86,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "Interval_ms": 10000,
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    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Trigger": "Software",
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  "FrameIndex": 87,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "Andor-Shutter TTL Value": "High to Open",
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  "WaitInterval": null,
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    "Interval_ms": 10000,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
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    "Positions": 2,
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "FileName": "img_000000087_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 87,
  "XYStage-Version": "Version: USB-9.2d ",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:44:43 -0700",
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  "COM1-Parity": "None",
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  "XYStage-Port": "COM3",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "FileName": "img_000000088_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-SerialResponse": "",
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  "FrameIndex": 88,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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    "Interval_ms": 10000,
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Shutter TTL Value": "High to Open",
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        "DeviceCoordinatesUm": {"XYStage": [
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000088_TRITC_000.tif",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 89,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "COM3-Handshaking": "Off",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "MicroManagerVersion": "1.4.20",
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    "Frames": 300,
    "BitDepth": 14,
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    "Positions": 2,
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000089_Phase (ETGFP)_000.tif",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000089_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 89,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:45:03 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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      {
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000089_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-90-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 90,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 90,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:45:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "GridColumn": 0,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
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  "XPositionUm": 11577.1,
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 90,
  "XYStage-Version": "Version: USB-9.2d ",
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  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000090_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 91,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 91,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:45:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "cb1e35a6-4ba1-4fc7-b197-d0ec3bcc8093",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 911476,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 91,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000091_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-91-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 91,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 91,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:45:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "ef2fa441-7ba5-4985-8492-c2826d10e750",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 911587,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 91,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000091_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-91-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 91,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Time": "2015-08-26 18:45:23 -0700",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000091_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 92,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:45:33 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 921639,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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      {
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        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000092_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-92-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 92,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 92,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:45:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "c72ac7b0-812f-4674-a7c9-e5e30f03a8fe",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 921752,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
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  "TITIRF-Mirror": "Out",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000093_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 93,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:45:43 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000093_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 94,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 94,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:45:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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        "GridColumnIndex": 0,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-JoyStick Reverse": "Off",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "FrameIndex": 94,
  "XYStage-Version": "Version: USB-9.2d ",
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  "Camera": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-95-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 95,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
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  "XYStage-Name": "XYStage",
  "NextFrame": 96,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:46:03 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 952060,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 95,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000095_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-96-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 96,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 96,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:46:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "6becb6da-bff9-4aaa-9e0d-8afa855f36c6",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 961623,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 96,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000096_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-96-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 96,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 96,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:46:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
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    "Frames": 300,
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  "Time": "2015-08-26 18:46:23 -0700",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000097_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "ROI": "0-0-1004-1002",
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  "Camera": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "FrameIndex": 98,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
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  "Andor-Region of Interest": "Full Image",
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  "TIFilterBlock1-State": "1",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000098_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 98,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:46:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 981595,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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      {
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000098_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 98,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:46:33 -0700",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "d2c59420-bb75-43ac-a9f2-d9dec0459a6b",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 982063,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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        "GridColumnIndex": 0,
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    "Frames": 300,
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
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  "COM3-Parity": "None",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Trigger": "Software",
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  "FileName": "img_000000100_Phase (ETGFP)_000.tif",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 100,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "COM4-Parity": "None",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:46:53 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "12507177-babf-40d7-978f-820643138aac",
  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 100,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000100_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 100,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "TILightPath-Label": "2-Left100",
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  "NextFrame": 101,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:46:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "cbf181a7-3570-4c64-9dd1-095744c7c5eb",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1002056,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 100,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000100_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-101-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 101,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 101,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:47:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "1ecb1d58-fa94-4d71-877f-501861b24278",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1011522,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    ],
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
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  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
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  "XYStage-StepSizeX_um": "0.0100",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 18:47:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000102_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 102,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:47:13 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "7d67c412-4307-48a7-bae0-fe786d13d489",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1021595,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000102_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-102-2-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 102,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 103,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:47:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "SlicesFirst": false,
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000103_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 103,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "COM4-Parity": "None",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "ROI": "0-0-1004-1002",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:47:23 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1032271,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000103_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-104-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 104,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:47:33 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "866fcefb-d5ee-489e-b78a-b8e323df8292",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1041472,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000104_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 104,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 104,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:47:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000104_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 104,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 105,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:47:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "f041343d-e145-4f49-b5f6-c05a4b87f37b",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1042073,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 104,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000104_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-105-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 105,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 105,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:47:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "caf14a25-8ae6-4bee-b2b5-42ed1f57a6c8",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1051480,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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    "Frames": 300,
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    "ComputerName": "vulcan_computer",
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    "Positions": 2,
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  "COM3-Name": "COM3",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "FileName": "img_000000106_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 106,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:47:53 -0700",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000106_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-107-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 107,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:48:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "7233b398-2730-44a3-ad3b-3b8ecf5c6d17",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1071647,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 107,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000107_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-107-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 107,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 107,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:48:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "efd3b543-60d8-4e61-9b0a-1be4765e9d22",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1071758,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-108-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 108,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 108,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:48:13 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 108,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000108_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 108,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 109,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:48:13 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "8d004663-952e-4a0a-a05c-498105287d14",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1082057,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 108,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000108_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-109-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 109,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 109,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:48:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
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        "GridColumnIndex": 0,
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    "Frames": 300,
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Trigger": "Software",
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  "FileName": "img_000000110_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 110,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-FanMode": "Full",
  "FrameIndex": 110,
  "XYStage-Version": "Version: USB-9.2d ",
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  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 111,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:48:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000110_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-111-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 111,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:48:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "9d530ffa-9b10-42b5-99ae-78cc0d61954f",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1111481,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000111_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-111-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 111,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 111,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:48:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "7fc8933a-c209-4f02-b2cf-1d0a6856ab27",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1111592,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 111,
  "Core-ChannelGroup": "Channel",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 112,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
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  "COM1-AnswerTimeout": "500.0000",
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  "Time": "2015-08-26 18:48:53 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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      1004,
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000112_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 112,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:48:53 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "7522b22a-e8bb-4898-bdb6-e318f6745b77",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1122067,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000112_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-113-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 113,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 113,
  "TILightPath-Name": "TILightPath",
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  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:03 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1131625,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 113,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000113_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-113-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 113,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 113,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "f15a2fda-a121-4ab2-b877-469653530e58",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1131737,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 113,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000113_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-113-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 113,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 114,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "3ee2476d-34f3-47dc-8a11-13f3da0d55b3",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1132206,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 113,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 114,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 115,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:13 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1142059,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 114,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000114_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-115-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 115,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 115,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "3373e0bb-4e41-4ee3-8aae-fdd5bfc58982",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1151476,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 115,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000115_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-115-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 115,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 115,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
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    "Frames": 300,
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  "FrameIndex": 116,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
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    "Frames": 300,
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    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000116_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 116,
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 116,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:33 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1161583,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 116,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000116_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-116-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 116,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 117,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "2afda767-ce5b-4774-b8ad-5227b2d4bbfd",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1162051,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000116_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-117-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 117,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:43 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000117_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 117,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "2f7fefdf-f53c-4aa5-b3f2-3ce26c597254",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1171753,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000117_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-117-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 117,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 118,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:49:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "e6442b78-f5be-4158-a59d-1d533ea3e012",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1172223,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ChColors": [
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    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
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  "Shutter-A-Name": "Shutter-A",
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  "FileName": "img_000000118_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "FrameIndex": 119,
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  "COM1-AnswerTimeout": "500.0000",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChContrastMax": [
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    "Positions": 2,
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000119_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 119,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:50:03 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000119_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 119,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 120,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:50:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "912f6d75-7c81-4c0f-90c5-70f73ae1dec5",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1192066,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000119_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-120-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 120,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 120,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:50:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "c95db270-f303-4612-bef8-f35f5ad21279",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1201636,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
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  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 18:50:23 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000121_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 121,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:50:23 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "b76749ec-b10e-451d-9444-10d0d8e74615",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1211592,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000121_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-121-2-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 121,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 122,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:50:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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    "Frames": 300,
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    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000122_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-Version": "Version: USB-9.2d ",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 123,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:50:33 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
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  "COM3-Handshaking": "Off",
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1222058,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 122,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000122_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-123-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 123,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 123,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "3f5e50ef-0d24-4398-9ca7-06d24e1c124d",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1231476,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000123_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-123-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 123,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
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  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000123_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 123,
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  "XYStage-Backlash-B": "0.0400",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:50:43 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
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  "ElapsedTime-ms": 1232058,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000123_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 124,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:50:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
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  "TIPFSStatus-Name": "TIPFSStatus",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "0d71357d-535a-40c2-a551-369fc7921a97",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1241627,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 125,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "COM1-AnswerTimeout": "500.0000",
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  "Time": "2015-08-26 18:51:03 -0700",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000125_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 125,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:51:03 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1251715,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000125_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 125,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000125_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 126,
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  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:51:13 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "BitDepth": 14,
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  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000126_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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"FrameKey-126-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 126,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "1ae99097-807d-4a96-8475-321e880a1150",
  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1261595,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Andor-Shutter Opening Time": "20",
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    "SlicesFirst": false,
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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    "Interval_ms": 10000,
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  "COM3-Parity": "None",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Trigger": "Software",
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  "Position": "Default",
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  "Andor-FanMode": "Full",
  "FrameIndex": 128,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-CoolerMode": "Fan off at shutdown",
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    "Frames": 300,
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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    "TimeFirst": false,
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Time": "2015-08-26 18:51:43 -0700",
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  "XYStage-Port": "COM3",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Shutter TTL Value": "High to Open",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000129_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 129,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "ElapsedTime-ms": 1291589,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "Interval_ms": 10000,
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    "Frames": 300,
    "BitDepth": 14,
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    "Positions": 2,
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "GridRow": 0,
    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 130,
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:51:53 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000130_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 130,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:51:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "4819116a-2e08-4b2a-9238-b82073a99e44",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Time": "2015-08-26 18:52:03 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000131_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 131,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:52:03 -0700",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1312223,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000131_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 132,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "Time": "2015-08-26 18:52:13 -0700",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000132_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 132,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:52:13 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "b461ef7d-e6aa-4be8-93ba-8f5980f939eb",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1321596,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000132_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-132-2-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 132,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 133,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:52:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "5dd9909d-87cc-49c2-bf9b-ecf847d39fde",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1322075,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
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  "TIFilterBlock1-Label": "3-TRITC",
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  "Andor-ActualInterval-ms": "138.19",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000133_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 134,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:52:33 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000134_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-134-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 134,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 134,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:52:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
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    "Frames": 300,
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    "IJType": 1,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:52:43 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Shutter TTL Value": "High to Open",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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    "SlicesFirst": false,
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    "Comment": "",
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    "GridColumn": 0,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000135_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 135,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "COM4-Parity": "None",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "Interval_ms": 10000,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Andor-FanMode": "Full",
  "FrameIndex": 136,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 136,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:52:53 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 136,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000136_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-136-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 136,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 136,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:52:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "ff20999f-e14b-4fda-b0f1-d69256da1034",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1361588,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 136,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000136_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-136-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 136,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 137,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:52:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "34b1dac5-1ec6-4401-9d67-b1241a6645b7",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000137_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 138,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:53:13 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1381625,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000138_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 138,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-TransposeCorrection": "0",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "FileName": "img_000000138_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 139,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "COM4-Parity": "None",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "Time": "2015-08-26 18:53:33 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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      1004,
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000140_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 140,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:53:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "2f0fb896-eb35-4261-a148-6ccab2f22f98",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000140_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-140-2-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 140,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "NextFrame": 141,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:53:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "FileName": "img_000000141_Phase (ETGFP)_000.tif",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "XYStage-SerialResponse": "",
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  "FrameIndex": 141,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-Error-E(nm)": "400.0000",
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  "COM4-Parity": "None",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:53:43 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "PixelAspect": 1,
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    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000141_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 142,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000142_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 142,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 142,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:53:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "c4ef8089-e30b-44c7-af87-66019664f952",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1421594,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 142,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
          11463.9,
          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
      }
    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
    ],
    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
      0,
      0,
      1004,
      1002
    ],
    "ChNames": [
      "Phase (ETGFP)",
      "ETGFP",
      "TRITC"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
      -1,
      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000142_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-142-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 142,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 143,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:53:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "e1b0c79f-f775-41ba-b36f-0a55df772f20",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1422131,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 142,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
      }
    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
    ],
    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
      "Phase (ETGFP)",
      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000142_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-143-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 143,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 143,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:54:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "6e393c92-7eab-42df-842e-d165065684c9",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
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    "Frames": 300,
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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    "Frames": 300,
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "Andor-FanMode": "Full",
  "FrameIndex": 144,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
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  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 145,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:54:13 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1442059,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 144,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000144_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-145-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 145,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 145,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:54:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "710dcfd6-b45d-4ae2-ad92-fee655c6c3e8",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1451611,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 145,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
    ],
    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
      "Phase (ETGFP)",
      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
      -1,
      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000145_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-145-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 145,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 145,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:54:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "0e71f42c-80e0-49ac-8936-d244495e95b0",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1451723,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 145,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 146,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
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  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Time": "2015-08-26 18:54:33 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000146_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 146,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:54:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1462076,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000146_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-147-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 147,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 147,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:54:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
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  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000147_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "ROI": "0-0-1004-1002",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:54:43 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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    "Interval_ms": 10000,
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000147_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 147,
  "XYStage-Version": "Version: USB-9.2d ",
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  "XYStage-Backlash-B": "0.0400",
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  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 148,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 149,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:54:53 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 148,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000148_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-149-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 149,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:55:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "865719f2-2b76-475a-a773-168d4c78c48a",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1491483,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 149,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000149_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-149-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 149,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 149,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:55:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "17d2a7b4-2185-4619-80aa-a3ed5d0085ee",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "GridColumn": 0,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-TransposeCorrection": "0",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:55:03 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
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    "Source": "Micro-Manager",
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000149_TRITC_000.tif",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 150,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 18:55:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000150_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 150,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 150,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:55:13 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1501590,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 150,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000150_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-150-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 150,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 151,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:55:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "0d323833-bd26-4e15-9473-baca5d84a825",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1502057,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 150,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000150_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-151-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 151,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 151,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:55:23 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000151_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-151-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 151,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 151,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:55:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "d962e2f4-2c58-4a9c-aed5-6743fb4b83c2",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1511591,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 151,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000151_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-151-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 151,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 152,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:55:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "031fb060-9fc0-4ee7-aadc-653bc9bc74b3",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1512060,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 151,
  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
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  "Shutter-A-Name": "Shutter-A",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 18:55:33 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000152_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 153,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:55:43 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "ElapsedTime-ms": 1531661,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000153_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-153-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 153,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 153,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:55:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 155,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 155,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:03 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1551620,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 155,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000155_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-155-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 155,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 155,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "596fa9bf-13ea-4f39-b204-258918c70c4d",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1551731,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 155,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000155_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-155-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 155,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 156,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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    "Frames": 300,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000156_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 156,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 157,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "1743b006-1f63-42f0-a27b-15bcd01b073e",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1562071,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 156,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
      }
    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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      "ETGFP",
      "TRITC"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000156_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-157-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 157,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 157,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "9d273160-f663-44ae-9db5-83e1706dbe4a",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1571476,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 157,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000157_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-157-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 157,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 157,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:23 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "48bac000-2058-42e3-b30e-2b37dfc35d01",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1571587,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 157,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000157_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-157-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 157,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 158,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "934a383b-69b9-48bb-9737-9fc27ad1902c",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1572096,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 157,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "ETGFP",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000157_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-158-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 158,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 158,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "9b75c1f5-0881-49d5-89dd-f58b20c1bd90",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1581484,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 158,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 159,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "Andor-ActualInterval-ms": "138.19",
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  "Time": "2015-08-26 18:56:43 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
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  "Shutter-A-Port": "COM1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000159_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 159,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:43 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1591753,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000159_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-159-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 159,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 160,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:56:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
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    "Frames": 300,
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  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Trigger": "Software",
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  "FrameIndex": 160,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-161-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 161,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 161,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:57:03 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1611605,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 161,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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      "ETGFP",
      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000161_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-161-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 161,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 162,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:57:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "cffd13c5-9574-4ce3-a2f2-935dc2683421",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1612097,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 161,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000161_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-162-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 162,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 162,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:57:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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        "GridColumnIndex": 0,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-Version": "Version: USB-9.2d ",
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
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    "ComputerName": "vulcan_computer",
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000162_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-163-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 163,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 163,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:57:23 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "28511a69-e33a-48bf-895a-190402f19724",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1631610,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 163,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000163_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-163-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 163,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 163,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:57:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "402d47c3-7b04-46fd-aa7c-a3a45c51900f",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1631723,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 163,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000163_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-163-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 163,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:57:23 -0700",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000163_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 164,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:57:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "99466f64-5b25-493c-8128-e5bfaab4b25d",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1641541,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000164_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-164-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 164,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 164,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:57:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "0adc606b-c11d-48c6-87ff-3686b1608768",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1641656,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XPositionUm": 11577.1,
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "GridRow": 0,
    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000165_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 165,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:57:43 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000165_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 166,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 166,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:57:53 -0700",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Time": "2015-08-26 18:30:13 -0700",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:57:53 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "Interval_ms": 10000,
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    "PositionIndex": 1,
    "SlicesFirst": false,
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    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000166_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 166,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-FanMode": "Full",
  "FrameIndex": 168,
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000168_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 168,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:58:13 -0700",
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  "COM1-Parity": "None",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "1",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "Comment": "",
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    "GridColumn": 0,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:58:13 -0700",
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  "XYStage-Port": "COM3",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "WaitInterval": null,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000168_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 169,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-Shutter": "Shutter-A",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000169_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 169,
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 169,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:58:23 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "ffff088c-72d2-4f9f-8fa4-bc8118c74e74",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1691736,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 169,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000169_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-169-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 169,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 170,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:58:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "4cccd015-6fb2-48ad-89d0-372cc04b5c07",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1692203,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000169_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-170-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 170,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 170,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:58:33 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 170,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000170_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-170-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 170,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 170,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:58:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "bd3651dc-84e2-4b45-8df9-64ba1531566a",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1701588,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 170,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "ETGFP",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000170_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-170-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 170,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 171,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:58:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "167f8627-2935-4e20-ab71-dd3046d29fb9",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1702071,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 170,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000171_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 172,
  "XYStage-Version": "Version: USB-9.2d ",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000172_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 172,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 172,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000172_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 172,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:58:53 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000172_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 173,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "a67903cd-3f98-4d22-904d-d5408cdf71bf",
  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1731651,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Andor-Shutter Opening Time": "20",
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000174_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "FrameIndex": 174,
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
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  "Time": "2015-08-26 18:59:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
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    "Interval_ms": 10000,
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "20.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000174_ETGFP_000.tif",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 174,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:59:13 -0700",
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  "COM1-Parity": "None",
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  "XYStage-Port": "COM3",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
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    "ComputerName": "vulcan_computer",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 175,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "IJType": 1,
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000175_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 176,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:59:23 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1752104,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 175,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000175_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-176-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 176,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 176,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "8a0115f2-9b0c-4118-88c8-22e61fe0d0b6",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1761645,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000176_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-176-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 176,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000176_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 176,
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:59:33 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000176_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-177-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 177,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 177,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:59:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "a4e5f375-5964-4548-ab24-99e6a9c9e26a",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1771484,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 178,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-AnswerTimeout": "500.0000",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "InitialPositionList": [
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
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    "Positions": 2,
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000178_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-FanMode": "Full",
  "FrameIndex": 178,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 18:59:53 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000178_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 179,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 179,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:00:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "a8000f59-eeef-41e9-9958-2a0232a55a90",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1791618,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 179,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000179_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-179-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 179,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 179,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:00:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "8796deb7-0026-4139-b624-dad659874fb2",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1791730,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 179,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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  "XPositionUm": 11577.1,
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  "FrameIndex": 181,
  "XYStage-Version": "Version: USB-9.2d ",
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  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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    "Frames": 300,
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    "IJType": 1,
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    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
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  "Time": "2015-08-26 19:00:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000182_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 182,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1821593,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000182_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 182,
  "XYStage-Version": "Version: USB-9.2d ",
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    "Frames": 300,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Trigger": "Software",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-FanMode": "Full",
  "FrameIndex": 183,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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    "Frames": 300,
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Time": "2015-08-26 19:00:53 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000184_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 184,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:00:53 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "50ca1f63-90b7-4d64-aaea-45dc5d89647b",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000184_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-185-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 185,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:03 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000185_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-185-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 185,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 185,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "e5d58e55-a98c-495f-b945-046734470961",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1851731,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 185,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000185_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-185-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 185,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 186,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "383038bb-3164-4343-a6c0-9c257cf4c7e8",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1852201,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 185,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 186,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "Andor-ActualInterval-ms": "138.19",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
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  "Time": "2015-08-26 19:01:13 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000186_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 187,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:23 -0700",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "ElapsedTime-ms": 1871476,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000187_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 187,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 187,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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      1002
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-TransposeCorrection": "0",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "TILightPath-Name": "TILightPath",
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  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
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  "Time": "2015-08-26 19:01:23 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "COM3-Parity": "None",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 188,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
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  "Camera": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000188_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 188,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 188,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:33 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1881587,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 188,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000188_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-188-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 188,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 189,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "09b6282c-c596-4950-b925-edc4ca2c3436",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1882052,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 188,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000188_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-189-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 189,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 189,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:43 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000189_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-189-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 189,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 189,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "63aa332a-3665-4235-a86b-5359f8c259c4",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1891594,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 189,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000189_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-189-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 189,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 190,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:01:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "5d165ce7-25a3-49f1-9b14-961f125f8915",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1892061,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 190,
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000190_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 191,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:02:03 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1911478,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000191_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-191-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 191,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
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  "AxisPositions": null,
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  "TIFilterBlock1-State": "1",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000191_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 191,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:02:03 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1912072,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000191_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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"FrameKey-192-0-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 192,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "674b2a31-6dc7-447f-b4a7-c0fcc582280c",
  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "WaitInterval": null,
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  "IntensiLightShutter-ND": "1",
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  "Andor-Shutter Opening Time": "20",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000193_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "FrameIndex": 193,
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Time": "2015-08-26 19:02:23 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
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  "Andor-Shutter Opening Time": "20",
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000193_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 193,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:02:23 -0700",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "2",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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    "Frames": 300,
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  "Andor-Trigger": "Software",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 194,
  "XYStage-Version": "Version: USB-9.2d ",
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    "IJType": 1,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "TILightPath-Label": "2-Left100",
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  "NextFrame": 195,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
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  "Time": "2015-08-26 19:02:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000194_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 195,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1951484,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
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      {
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
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    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000195_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 195,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 195,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:02:43 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
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  "Core-Camera": "Andor",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 195,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000195_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 195,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 196,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:02:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "9ec2441f-268f-4871-89f5-ef19f95eab70",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1952084,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 195,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000195_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-196-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 196,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 196,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:02:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "10742100-6d55-4f6e-8607-14af53b9845c",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1961478,
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000197_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
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  "FrameIndex": 197,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000197_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 197,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TIPFSOffset-Position": "69.224999999999994",
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  "TIFilterBlock1-State": "2",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000197_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 198,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:03:13 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1981522,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000198_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-198-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 198,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 198,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:03:13 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "2159db3b-e97d-4621-8e3d-acbacd14df1c",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 1981635,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
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  "FileName": "img_000000199_ETGFP_000.tif",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Frames": 300,
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    "ComputerName": "vulcan_computer",
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "FileName": "img_000000199_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-OptAcquireMode Description": "",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Andor-FanMode": "Full",
  "FrameIndex": 200,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
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  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
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  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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        "GridColumnIndex": 0,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-JoyStick Reverse": "Off",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "FileName": "img_000000200_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 200,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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    "SlicesFirst": false,
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
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  "Andor-Description": "Andor camera adapter",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000201_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
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  "Andor-ReadoutMode": "35.000 MHz",
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  "FrameIndex": 201,
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  "Camera": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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    "Frames": 300,
    "BitDepth": 14,
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    "Positions": 2,
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "Andor-FanMode": "Full",
  "FrameIndex": 201,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 202,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:03:43 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000201_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 202,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:03:53 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "7c2e85f6-677a-4445-90f4-417ffd21a71f",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2021477,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 202,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000202_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-202-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 202,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 202,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:03:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "ba27b090-dfa6-49d7-9446-0a3a7117f9fd",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "Date": "2015-08-26",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000203_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 203,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000203_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 204,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "NextFrame": 204,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:04:13 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2041626,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 204,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000204_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-204-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 204,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 204,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:04:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "c55575ec-4c27-4806-ab57-3b64cc561cab",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2041739,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 204,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000204_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-204-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 204,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 205,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:04:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "603c3a2c-c95d-4647-8dcf-5c013e3d9471",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2042209,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 204,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
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  "XYStage-Version": "Version: USB-9.2d ",
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  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
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    "Width": 1004,
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      1004,
      1002
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    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000206_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "ROI": "0-0-1004-1002",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:04:33 -0700",
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  "Slice": 0,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000206_TRITC_000.tif",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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"FrameKey-207-0-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 207,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "Shutter-A",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "ChNames": [
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    "Frames": 300,
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 208,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Time": "2015-08-26 19:04:53 -0700",
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  "XYStage-Port": "COM3",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000208_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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"FrameKey-208-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 208,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 208,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:04:53 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "2ebe4dcf-f9c8-4af3-963c-42da1e759782",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2081595,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000208_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-208-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 208,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 209,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:04:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "80156253-d478-41e7-a702-c9fd364d0b62",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-FanMode": "Full",
  "FrameIndex": 210,
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
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    "ComputerName": "vulcan_computer",
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    "ChContrastMax": [
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    "Positions": 2,
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000210_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 210,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 19:05:13 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "1",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000210_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 210,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:05:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "85a46b6f-8b9b-47e1-97b1-a66057d90bd6",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2102062,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000210_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-211-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 211,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 211,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "508bdf3c-6c6e-4e9c-b0d4-b43ae4f3ba60",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2111622,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 19:05:33 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "Slice": 0,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000212_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Andor-FanMode": "Full",
  "FrameIndex": 212,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:05:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "f58eda14-949c-4c41-8df9-6dd4ec21061d",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2121635,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000212_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-212-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 212,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 213,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:05:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "FileName": "img_000000213_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 213,
  "XYStage-Version": "Version: USB-9.2d ",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "COM4-Parity": "None",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Time": "2015-08-26 19:05:53 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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      0,
      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000214_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 214,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:05:53 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
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  "COM3-AnswerTimeout": "500.0000",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000214_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-215-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 215,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 215,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:06:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000215_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-216-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 216,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 216,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:06:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "9fe4c2ce-6a5f-4874-b840-c38ba119761b",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2161485,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 216,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
      }
    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
      -1,
      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000216_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-216-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 216,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 216,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:06:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "3745f0b3-c023-4bc9-a59a-e53b034458ba",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2161597,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 216,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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          -802.2
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
    ],
    "ChNames": [
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      "ETGFP",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000216_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-216-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 216,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 19:06:13 -0700",
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  "TIFilterBlock1-State": "2",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
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  "TITIRF-Mirror": "Out",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000216_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 217,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:06:23 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2171648,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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      {
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000217_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 217,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:06:23 -0700",
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  "COM1-Parity": "None",
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  "XYStage-Port": "COM3",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "d55b1e7d-0d59-4125-ac52-8929aa6d2251",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2171760,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XPositionUm": 11577.1,
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Width": 1004,
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "GridColumn": 0,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000218_ETGFP_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 218,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:06:33 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000218_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 219,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 219,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:06:43 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 220,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:06:53 -0700",
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  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000220_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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"FrameKey-221-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 221,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 221,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:07:03 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "9a08b5c8-5e3e-452f-9178-e91de9d77a73",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2211621,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 221,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000221_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-221-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 221,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 221,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:07:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000222_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 222,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 222,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:07:13 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "7690f33f-ed97-488f-a05c-d29a65f4ad05",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2221590,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 222,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000222_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-222-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 222,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 223,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:07:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "71d6c925-3d34-4d88-bc64-efc6430b6942",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2222056,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 222,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000222_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-223-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 223,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
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  "Time": "2015-08-26 19:07:23 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000223_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 223,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:07:23 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "9775e48d-b74c-4892-899a-64b3941835c6",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2231598,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000223_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-223-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 223,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 224,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:07:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "b942f17f-68b5-4d1d-8963-09f8d2b8aa2b",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2232089,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 224,
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
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  "XYStage-Error-E(nm)": "400.0000",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 19:07:33 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000224_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 225,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:07:43 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "cfc3122b-a3c2-490e-bd3b-7552364a28da",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2251629,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000225_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-225-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 225,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 225,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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    "SlicesFirst": false,
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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    "SlicesFirst": false,
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-Trigger": "Software",
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  "Position": "Default",
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  "XYStage-SerialResponse": "",
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  "FrameIndex": 226,
  "XYStage-Version": "Version: USB-9.2d ",
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  "Camera": "",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
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  "Andor-Region of Interest": "Full Image",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 19:08:03 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000227_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 227,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:08:03 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "2e8b1008-8aca-4386-bcbe-bead4b860519",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2271599,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 227,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000227_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-227-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 227,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 228,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:08:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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        "GridColumnIndex": 0,
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    "Frames": 300,
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XPositionUm": 11577.1,
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  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "FrameIndex": 228,
  "XYStage-Version": "Version: USB-9.2d ",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "IJType": 1,
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "TimeFirst": false,
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "100.00",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000228_TRITC_000.tif",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-FanMode": "Full",
  "FrameIndex": 229,
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Trigger": "Software",
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  "Andor-FanMode": "Full",
  "FrameIndex": 229,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
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  "XYStage-Error-E(nm)": "400.0000",
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  "Time": "2015-08-26 19:08:23 -0700",
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  "TIFilterBlock1-State": "1",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000229_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 229,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:08:23 -0700",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2292057,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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      0,
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000229_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-230-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 230,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:08:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
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  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "974abe11-1df2-44a4-a0a4-8041f30f90c0",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2301481,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 231,
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
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  "COM1-AnswerTimeout": "500.0000",
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  "Time": "2015-08-26 19:08:43 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
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  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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      0,
      1004,
      1002
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000231_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
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  "Andor-FanMode": "Full",
  "FrameIndex": 231,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 231,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:08:43 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "e4d526ae-5ed6-488a-b37c-16b54cafc19d",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2311735,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "PositionName": "Pos1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
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  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000231_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-231-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 231,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 232,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
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      1004,
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    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000231_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 232,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:08:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "44643909-b64e-4c74-84e5-d60249053d27",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2321625,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000232_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-232-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 232,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 232,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:08:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "2e6270c6-6105-46e1-aa33-93c401db7877",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2321738,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 233,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
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  "COM1-AnswerTimeout": "500.0000",
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  "XYStage-Error-E(nm)": "400.0000",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Time": "2015-08-26 19:09:03 -0700",
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  "TIFilterBlock1-State": "1",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000233_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 233,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Acceleration-AC(ms)": "100",
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  "TILightPath-Label": "2-Left100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:09:03 -0700",
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  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
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  "TINosePiece-Name": "TINosePiece",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "00aba107-f9e4-4ad6-a167-8b711fba13f8",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2332053,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000233_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-234-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 234,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
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  "XYStage-Name": "XYStage",
  "NextFrame": 234,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:09:13 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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    "Frames": 300,
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    "TimeFirst": false,
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-FanMode": "Full",
  "FrameIndex": 234,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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    "IJType": 1,
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    "Comment": "",
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    "Prefix": "082615_1",
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    "Frames": 300,
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    "KeepShutterOpenChannels": false,
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    "ComputerName": "vulcan_computer",
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "XYStage-JoyStick Reverse": "Off",
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  "Time": "2015-08-26 19:09:23 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Shutter TTL Value": "High to Open",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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        "DeviceCoordinatesUm": {"XYStage": [
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    "SlicesFirst": false,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000235_Phase (ETGFP)_000.tif",
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  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 235,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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    "Positions": 2,
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "Andor-Trigger": "Software",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 235,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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        "Label": "Pos1",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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      1004,
      1002
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Shutter": "Open",
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  "COM4-Handshaking": "Off",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000235_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
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  "Andor-FanMode": "Full",
  "FrameIndex": 236,
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Shutter Closing Time": "20",
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  "AxisPositions": null,
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  "Time": "2015-08-26 19:09:33 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
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    "Comment": "",
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    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
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    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000236_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 236,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "XYStage-Name": "XYStage",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "COM1-Parity": "None",
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  "Andor-Isolated Crop Mode": "Off",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "49c4cae7-0092-45c3-9c05-22c1669e230e",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2361750,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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  "Andor-Shutter TTL Value": "High to Open",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "Label": "Pos1",
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    "PixelType": "GRAY16",
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000237_ETGFP_000.tif",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Camera": "",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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    "InitialPositionList": [
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    "Interval_ms": 10000,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "Positions": 2,
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000237_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
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  "Andor-OptAcquireMode Description": "",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 238,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Label": "2-Left100",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:09:53 -0700",
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  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
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  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 238,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
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  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000238_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-238-1-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 238,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 238,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:09:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "fba87394-2075-4d89-b9b2-11953b95eacc",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2381723,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 238,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000238_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-238-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 238,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 239,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:09:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "40e80eda-bde5-404f-a38d-f3b7e83ad84e",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2382192,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 238,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
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    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "SlicesFirst": false,
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    "MicroManagerVersion": "1.4.20",
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    "Frames": 300,
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 240,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      1002
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
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  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "AxisPositions": null,
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  "Time": "2015-08-26 19:10:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "COM3-Parity": "None",
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  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 241,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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    "Interval_ms": 10000,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
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    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
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  "IntensiLightShutter-Version": "V1.00",
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  "XYStage-JoyStick Reverse": "Off",
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  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "TILightPath-Name": "TILightPath",
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  "Time": "2015-08-26 19:10:23 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "SlicesFirst": false,
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
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    "Positions": 2,
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000241_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 241,
  "XYStage-Version": "Version: USB-9.2d ",
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  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
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  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
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  "AxisPositions": null,
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "COM3-AnswerTimeout": "500.0000",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "UUID": "7b87750f-edc7-4362-a836-c694a91ee124",
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  "XYStage-JoyStick Fast Speed": "60",
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  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2412060,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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  "TITIRF-Mirror": "Out",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "Positions": 2,
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
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  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000241_TRITC_000.tif",
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},
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 242,
  "XYStage-Version": "Version: USB-9.2d ",
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  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
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  "Time": "2015-08-26 19:10:33 -0700",
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  "Andor-CCDTemperatureSetPoint": "-70",
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  "TIFilterBlock1-State": "1",
  "Slice": 0,
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
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    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000242_Phase (ETGFP)_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "ETGFP",
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  "Shutter-A-State": "0",
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  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 242,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
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  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
  "XYStage-StepSizeY_um": "0.0100",
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  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:10:33 -0700",
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  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "TIPFSOffset-Name": "TIPFSOffset",
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  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
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  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
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  "Core-TimeoutMs": "5000",
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  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2421742,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
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  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
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    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
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    "ChNames": [
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000242_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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},
"FrameKey-242-2-0": {
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  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 242,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 243,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:10:33 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "0b447ff1-f7fa-4a4e-aaa0-f06264df3a34",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2422210,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
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    "Frames": 300,
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  "XPositionUm": 11577.1,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "Height": 1002,
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "Label": "Pos1",
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    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
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    "SlicesFirst": false,
    "ChContrastMin": [
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    "Comment": "",
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    "Frames": 300,
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    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChColors": [
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
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  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000243_TRITC_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "SliceIndex": 0,
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  "FrameIndex": 244,
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  "TIFilterBlock1-Label": "2-ETGFP",
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  "Andor-ActualInterval-ms": "138.19",
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "AxisPositions": null,
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
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    "InitialPositionList": [
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "SlicesFirst": false,
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    "MicroManagerVersion": "1.4.20",
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    "Comment": "",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000244_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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},
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 244,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "58.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 20,
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  "NextFrame": 244,
  "TILightPath-Name": "TILightPath",
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  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:10:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
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  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
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  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 1,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
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  "Core-Shutter": "IntensiLightShutter",
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  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2441588,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 244,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
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    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "TRITC"
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000244_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
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"FrameKey-244-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 244,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
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  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 245,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:10:53 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "a219bc90-efd4-42c9-abab-6d2d63de4c20",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2442096,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 244,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
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      {
        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
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    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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      0,
      0
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    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
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      0,
      1004,
      1002
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    "ChNames": [
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      "ETGFP",
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    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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      -13369600,
      -65536
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    "TimeFirst": false,
    "ChContrastMax": [
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      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000244_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-245-0-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "Phase (ETGFP)",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 245,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "2-ETGFP",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 245,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:11:03 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "1",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 0,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "Shutter-A",
  "TILightPath-State": "1",
  "UUID": "093db2de-a8e9-4186-b93e-981885dcd9b6",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2451620,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 245,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
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  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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          -845.7
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "XYStage-CompileDate": "May 30 2013:16:54:11",
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
        "Label": "Pos0",
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        "DeviceCoordinatesUm": {"XYStage": [
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        "GridColumnIndex": 0,
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    "PixelType": "GRAY16",
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    "Date": "2015-08-26",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "Width": 1004,
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    "Comment": "",
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    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
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  "Andor-ReadoutTime": "38.19",
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  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
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  "PixelType": "GRAY16",
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  "COM1-DelayBetweenCharsMs": "0.0000",
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  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
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  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000246_Phase (ETGFP)_000.tif",
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  "XYStage-JoyStick Reverse": "Off",
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  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
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  "Andor-OptAcquireMode Description": "",
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  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "Andor-FanMode": "Full",
  "FrameIndex": 246,
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  "ROI": "0-0-1004-1002",
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  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
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  "Andor-CoolerMode": "Fan off at shutdown",
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  "AxisPositions": null,
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  "Time": "2015-08-26 19:11:13 -0700",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "TIScope-SoftwareVersion": "4.4.1.728",
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  "XYStage-JoyStick Fast Speed": "60",
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  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
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  "Andor-Shutter TTL Value": "High to Open",
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  "Width": 1004,
  "WaitInterval": null,
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  "TIScope-FirmwareVersion": "Ti-E V1.30",
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  "Andor-Shutter Opening Time": "20",
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    "InitialPositionList": [
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        "GridColumnIndex": 0,
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    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
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    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
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    "MicroManagerVersion": "1.4.20",
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    "GridRow": 0,
    "Comment": "",
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    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
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    "ComputerName": "vulcan_computer",
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    "KeepShutterOpenSlices": false,
    "ChColors": [
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    "TimeFirst": false,
    "ChContrastMax": [
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "COM3-Name": "COM3",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Description": "Andor camera adapter",
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  "Andor-Exposure": "20.00",
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  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
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  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
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  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000246_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
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  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
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  "Andor-ReadoutMode": "35.000 MHz",
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  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 246,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
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  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
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  "NextFrame": 247,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:11:13 -0700",
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  "COM1-Parity": "None",
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  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
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  "XYStage-SerialCommand": "",
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  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
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    "Frames": 300,
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  "IntensiLightShutter-Port": "COM4",
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  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
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  "TIScope-DeviceAddress": "USB_DEVICE(1)",
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  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
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  "FrameIndex": 247,
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    "MicroManagerVersion": "1.4.20",
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    "IJType": 1,
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    "Prefix": "082615_1",
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    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
      -1,
      -13369600,
      -65536
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
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    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Focusing",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "20.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000247_ETGFP_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
},
"FrameKey-247-2-0": {
  "Core-Focus": "TIPFSOffset",
  "Andor-ReadoutMode": "35.000 MHz",
  "Channel": "TRITC",
  "Core-Initialize": "1",
  "Shutter-A-State": "0",
  "COM1-Verbose": "0",
  "TIPFSStatus-FullFocusWaitAfterLockMs": "0.0000",
  "XYStage-SerialResponse": "",
  "Andor-FanMode": "Full",
  "FrameIndex": 247,
  "XYStage-Version": "Version: USB-9.2d ",
  "XYStage-BuildName": "STD_XY",
  "COM1-Description": "Serial port driver (boost:asio)",
  "TIFilterBlock1-Label": "3-TRITC",
  "Andor-BaselineClamp": "Enabled",
  "XYStage-Backlash-B": "0.0400",
  "Andor-ActualInterval-ms": "138.19",
  "XYStage-Speed-S": "5.7459",
  "ROI": "0-0-1004-1002",
  "Andor-PixelType": "16bit",
  "COM1-AnswerTimeout": "500.0000",
  "Camera": "",
  "Andor-Region of Interest": "Full Image",
  "XYStage-Error-E(nm)": "400.0000",
  "XYStage-StepSizeX_um": "0.0100",
  "COM4-Parity": "None",
  "Core-ImageProcessor": "",
  "Andor-Advanced | Snap Image Timing Mode": "Delay for Exposure",
  "SlicePosition": null,
  "Andor-CoolerMode": "Fan off at shutdown",
  "Exposure-ms": 100,
  "XYStage-StepSizeY_um": "0.0100",
  "COM1-DataBits": "8",
  "TIPFSOffset-Position": "69.224999999999994",
  "Core-AutoShutter": "1",
  "XYStage-Acceleration-AC(ms)": "100",
  "Shutter-A-Mode": "Fast",
  "TILightPath-Label": "2-Left100",
  "Andor-Pre-Amp-Gain": "3.8x",
  "XYStage-Name": "XYStage",
  "NextFrame": 248,
  "TILightPath-Name": "TILightPath",
  "Andor-Shutter Closing Time": "20",
  "Andor-EMSwitch": "On",
  "Core-XYStage": "XYStage",
  "AxisPositions": null,
  "Andor-1. Camera Information : | Type | Model | Serial No. |": "| iXon | DU8285_VP | 4497 |",
  "Time": "2015-08-26 19:11:23 -0700",
  "IntensiLightShutter-State": "0",
  "COM1-Parity": "None",
  "COM4-Name": "COM4",
  "Andor-Isolated Crop Mode": "Off",
  "COM4-BaudRate": "9600",
  "XYStage-Port": "COM3",
  "Andor-CCDTemperatureSetPoint": "-70",
  "TINosePiece-Label": "5-Plan Apo 100x NA 1.40 Oil",
  "Core-AutoFocus": "TIPFSStatus",
  "COM1-StopBits": "1",
  "XYStage-SerialCommand": "",
  "TIPFSOffset-Name": "TIPFSOffset",
  "TIScope-DeviceIndex": "1",
  "BitDepth": 14,
  "TIFilterBlock1-State": "2",
  "Slice": 0,
  "TIPFSStatus-Name": "TIPFSStatus",
  "XYStage-JoyStick Slow Speed": "5",
  "COM4-Verbose": "0",
  "COM3-AnswerTimeout": "500.0000",
  "Andor-KeepCleanTime": "0.00",
  "Shutter-A-Controller Info": "SC-v1.12S-",
  "TIFilterBlock1-ExtraDelayMs": "0.0000",
  "TINosePiece-Name": "TINosePiece",
  "TINosePiece-State": "4",
  "TIScope-DriverVersion": "micusb.sys 3.0.1.9",
  "Andor-Camera": "| iXon | DU8285_VP | 4497 |",
  "ChannelIndex": 2,
  "TIScope-SoftwareVersion": "4.4.1.728",
  "COM3-Handshaking": "Off",
  "Andor-Gain": "3",
  "Core-Shutter": "IntensiLightShutter",
  "TILightPath-State": "1",
  "UUID": "24302b5a-67db-4b51-bc08-115d939b12ff",
  "Core-Camera": "Andor",
  "Core-TimeoutMs": "5000",
  "Core-SLM": "",
  "XYStage-JoyStick Fast Speed": "60",
  "Shutter-A-NDSetting": "1",
  "XYStage-Maximum Speed (Do Not Change)": "8.5760",
  "ElapsedTime-ms": 2472110,
  "TIPFSStatus-FullFocusTimeoutMs": "5000.0000",
  "COM1-Handshaking": "Off",
  "TIPFSStatus-State": "On",
  "Andor-Shutter TTL Value": "High to Open",
  "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter",
  "Frame": 247,
  "Core-ChannelGroup": "Channel",
  "PositionIndex": 1,
  "Width": 1004,
  "WaitInterval": null,
  "TITIRF-Mirror": "Out",
  "Shutter-A-Port": "COM1",
  "TIFilterBlock1-Name": "TIFilterBlock1",
  "IntensiLightShutter-Name": "IntensiLightShutter",
  "COM3-StopBits": "1",
  "PositionName": "Pos1",
  "TINosePiece-ExtraDelayMs": "0.0000",
  "PixelSizeUm": 0.08,
  "Height": 1002,
  "Andor-TransposeMirrorX": "0",
  "XYStage-CompileDate": "May 30 2013:16:54:11",
  "IntensiLightShutter-ND": "1",
  "COM3-Verbose": "1",
  "TIScope-FirmwareVersion": "Ti-E V1.30",
  "Andor-TransposeMirrorY": "0",
  "Andor-Shutter Opening Time": "20",
  "Summary": {
    "Slices": 1,
    "InitialPositionList": [
      {
        "DeviceCoordinatesUm": {"XYStage": [
          11463.9,
          -845.7
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        "GridColumnIndex": 0,
        "Label": "Pos0",
        "GridRowIndex": 0
      },
      {
        "DeviceCoordinatesUm": {"XYStage": [
          11577.1,
          -802.2
        ]},
        "GridColumnIndex": 0,
        "Label": "Pos1",
        "GridRowIndex": 0
      }
    ],
    "Interval_ms": 10000,
    "UUID": "c857d548-4068-4b84-9064-da40c1cec55c",
    "UserName": "cellshape",
    "Depth": 2,
    "PixelType": "GRAY16",
    "Time": "2015-08-26 18:30:13 -0700",
    "Date": "2015-08-26",
    "z-step_um": 0,
    "MetadataVersion": 10,
    "PositionIndex": 1,
    "SlicesFirst": false,
    "ChContrastMin": [
      0,
      0,
      0
    ],
    "Width": 1004,
    "PixelAspect": 1,
    "MicroManagerVersion": "1.4.20",
    "ROI": [
      0,
      0,
      1004,
      1002
    ],
    "ChNames": [
      "Phase (ETGFP)",
      "ETGFP",
      "TRITC"
    ],
    "IJType": 1,
    "GridRow": 0,
    "Comment": "",
    "Height": 1002,
    "GridColumn": 0,
    "Prefix": "082615_1",
    "PixelSize_um": 0.08,
    "Frames": 300,
    "BitDepth": 14,
    "KeepShutterOpenChannels": false,
    "Source": "Micro-Manager",
    "Channels": 3,
    "ComputerName": "vulcan_computer",
    "CustomIntervals_ms": [],
    "KeepShutterOpenSlices": false,
    "ChColors": [
      -1,
      -13369600,
      -65536
    ],
    "TimeFirst": false,
    "ChContrastMax": [
      65536,
      65536,
      65536
    ],
    "Positions": 2,
    "Directory": "D:\\Rico Vulcan\\082615"
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  "Source": "Andor",
  "XYStage-OverShoot(um)": "0.0000",
  "XYStage-Wait_Cycles": "0",
  "COM4-StopBits": "1",
  "COM3-Name": "COM3",
  "IntensiLightShutter-Port": "COM4",
  "Andor-Binning": "1",
  "Andor-FrameTransfer Help": "Should only turn on FrameTransfer if using Burst or Live mode.",
  "COM4-DataBits": "8",
  "TIPFSStatus-Status": "Locked in focus",
  "TIScope-DeviceAddress": "USB_DEVICE(1)",
  "COM3-DataBits": "8",
  "XYStage-MotorOnOff": "On",
  "XYStage-Description": "ASI XY stage driver adapter",
  "TITIRF-Name": "TITIRF",
  "Andor-Shutter": "Open",
  "Binning": "1",
  "Andor-ReadoutTime": "38.19",
  "COM4-Handshaking": "Off",
  "Andor-FrameTransfer": "Off",
  "Andor-Description": "Andor camera adapter",
  "COM3-DelayBetweenCharsMs": "0.0000",
  "PixelType": "GRAY16",
  "Andor-TransposeXY": "0",
  "COM1-DelayBetweenCharsMs": "0.0000",
  "COM4-DelayBetweenCharsMs": "0.0000",
  "Andor-Advanced | Snap Image Additional Delay (ms)": "0",
  "TILightPath-ExtraDelayMs": "0.0000",
  "Andor-Exposure": "100.00",
  "Andor-TransposeCorrection": "0",
  "COM4-AnswerTimeout": "500.0000",
  "Andor-CCDTemperature Help": "Wait for temperature to stabilize before acquisition.",
  "COM4-Description": "Serial port driver (boost:asio)",
  "COM3-Description": "Serial port driver (boost:asio)",
  "YPositionUm": -802.2,
  "COM3-Parity": "None",
  "COM1-BaudRate": "9600",
  "Andor-VerticalSpeed": "3.73",
  "Shutter-A-Description": "Sutter Lambda shutter adapter",
  "XYStage-TransposeMirrorY": "0",
  "XPositionUm": 11577.1,
  "XYStage-TransposeMirrorX": "0",
  "Andor-TimeOut": "10000",
  "Andor-Trigger": "Software",
  "Shutter-A-Name": "Shutter-A",
  "IntensiLightShutter-Version": "V1.00",
  "FileName": "img_000000247_TRITC_000.tif",
  "Andor-CCDTemperature": "24",
  "TITIRF-Position": "0",
  "Core-Galvo": "",
  "XYStage-JoyStick Reverse": "Off",
  "COM3-BaudRate": "9600",
  "Position": "Default",
  "XYStage-OnlySendSerialCommandOnChange": "Yes",
  "Andor-VerticalClockVoltage": "Normal",
  "Andor-OptAcquireMode Description": "",
  "COM1-Name": "COM1",
  "CameraChannelIndex": 0,
  "SliceIndex": 0,
  "XYStage-FinishError-PCROS(nm)": "24.0000",
  "ZPositionUm": 69.225
}
}