{ "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "FrameKey-0-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 0, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 0, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:24:54 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "f2df1a1f-ab4f-47c0-b584-23117e03d226", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 45066, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 0, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000000_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-0-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 0, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 1, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:24:55 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "c6280f02-8512-406c-8a45-454d1a25c43f", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 46237, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 0, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000000_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-1-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 1, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 2, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:25:31 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "75a9eae4-93b9-45f6-b968-7cdeb3c6d8cd", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 81256, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 1, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000001_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-2-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 2, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 3, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:26:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "903f4048-87b0-4424-a554-88d5f11b94d3", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 135083, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 2, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000002_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-3-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 3, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 4, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:27:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "38415472-1370-4da5-93cb-297d9d6f0343", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 194922, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 3, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000003_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-4-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 4, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 4, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:28:53 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "37d17fb8-caea-4c2e-93da-4bb42a2a3865", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 283589, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 4, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000004_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-4-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 4, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 5, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:28:55 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "23bfce21-3d50-47bc-b056-590fd5cf5279", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 285309, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 4, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000004_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-5-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 5, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 6, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:29:35 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "7b2a62d0-e093-49a7-bc5a-4a50d09d9842", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 325378, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 5, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000005_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-6-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 6, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 7, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:30:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "55b1a979-56d9-48b6-84d4-17909fb5dd8f", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 374976, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 6, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000006_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-7-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 7, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 8, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:31:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "22c01405-a164-41e5-915c-ac9b06cd3f47", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 435005, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 7, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000007_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-8-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 8, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 8, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:32:58 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "4e5e6dd2-9fe2-4555-99ec-17148c9e54b9", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 528552, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 8, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000008_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-8-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 8, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 9, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:32:59 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "33995056-76ca-49e3-b054-d3862b459f71", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 529952, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 8, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000008_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-9-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 9, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 10, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:33:41 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "b2519da3-92ab-450c-80e9-faff60d87f1d", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 572180, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 9, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000009_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-10-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 10, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 11, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:34:29 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "f57c8949-c6d5-4ca8-963e-f9102472b30d", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 619559, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 10, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000010_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-11-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 11, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 12, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:35:27 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "1f145136-a900-4028-8ebf-27bd871517f2", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 677407, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 11, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000011_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-12-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 12, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 12, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:37:00 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "f52c75c7-4500-46ed-b10b-2ae9a972269b", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 770894, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 12, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000012_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-12-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 12, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 13, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:37:02 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "fe2b9c40-1b40-4346-8c2e-4d8cef65e43d", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 772255, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 12, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000012_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-13-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 13, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 14, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:37:45 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "313ad19d-f0e3-47c0-8150-a2545a6b3d57", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 815254, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 13, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000013_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-14-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 14, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 15, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:38:31 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "98dcc9f5-13bc-4dc4-ae9f-4ddd80309aee", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 861332, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 14, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000014_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-15-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 15, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 16, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:39:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "def9f5e1-a12a-4958-9eb4-78faf578a93a", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 914970, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 15, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000015_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-16-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 16, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 16, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:41:00 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "a3230503-a42f-4a4f-be37-0106be6b7ff3", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1010747, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 16, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000016_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-16-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 16, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 17, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:41:01 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "6af44835-68fc-4e6f-bae7-f5596621fe6a", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1012057, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 16, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000016_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-17-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 17, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 18, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:41:43 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "39925384-9647-42be-8190-356a45a2496b", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1053997, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 17, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000017_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-18-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 18, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 19, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:42:30 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "1601ddbc-773f-42a8-8891-2593f403e65b", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1101005, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 18, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000018_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-19-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 19, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 20, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:43:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "4325a0f8-062e-4656-a4db-9f4d55deb852", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1155023, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 19, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000019_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-20-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 20, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 20, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:44:58 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "44497765-ac93-447f-a8d7-52d1dca8f41a", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1248390, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 20, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000020_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-20-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 20, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 21, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:44:59 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "f6145bd3-1ef6-4b08-b87f-dd38d8ac6c97", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1249731, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 20, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000020_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-21-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 21, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 22, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:45:43 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "d734c78d-a21d-4591-9b2c-fd0a96e5c1b5", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1294109, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 21, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000021_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-22-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 22, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 23, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:46:27 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "5bf1a47c-2b34-4969-be47-57ecbf462248", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1337638, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 22, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000022_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-23-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 23, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 24, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:47:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "1a6124fb-2012-4579-b83a-c45aa0d0a079", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1395086, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 23, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000023_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-24-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 24, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 24, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:48:57 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "7236327f-8ae7-4fbf-bb8e-c636b59be694", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1487593, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 24, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000024_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-24-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 24, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 25, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:48:58 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "291b2ff5-dc4a-44f7-a541-540e1ecc42e6", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1489024, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 24, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000024_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-25-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 25, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 26, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:49:40 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "893fbf8d-d50f-48c3-b063-19ce641e2d13", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1530452, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 25, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000025_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-26-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 26, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 27, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:50:27 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "82949ede-cbf8-4faa-a1b8-9a98c3cd13ad", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1578180, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 26, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000026_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-27-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 27, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 28, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:51:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "8c89283f-3ebc-4263-b58a-d072dc13b046", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1634929, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 27, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000027_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-28-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 28, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 28, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:53:00 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "f33e7f8c-1340-4502-820d-d8832e30adc9", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1731086, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 28, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000028_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-28-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 28, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 29, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:53:02 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "7a00c366-bc1a-4e58-a2aa-ce3c19137cb9", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1732416, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 28, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000028_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-29-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 29, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 30, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:53:44 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "a6beeec8-5138-45c5-a9f1-410231055f16", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1774295, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 29, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000029_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-30-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 30, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 31, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:54:32 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "0854f7c3-a3c1-4090-b96b-24bdc0f3ba14", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1822504, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 30, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000030_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-31-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 31, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 32, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:55:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "6be80c3c-c6d4-4367-b01a-0e2032fa0e4a", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1874902, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 31, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000031_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-32-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 32, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 32, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:56:59 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "3083af05-8252-45ef-9501-eb7bf3816cf7", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1969749, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 32, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000032_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-32-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 32, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 33, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:57:01 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "05d6da8e-4464-4d18-b4d0-024b7fda4ae5", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 1971219, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 32, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000032_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-33-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 33, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 34, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:57:45 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "a5805f4c-ef23-4fcc-9823-cc2a817abd65", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2015337, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 33, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000033_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-34-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 34, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 35, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:58:33 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "7ea11b0f-30cd-47f6-8853-80c308cdd823", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2063306, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 34, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000034_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-35-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 35, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 36, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 18:59:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "b84c5ab0-9dd8-417e-a707-cc3de5fb30f1", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2114765, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 35, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000035_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-36-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 36, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 36, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:01:02 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "27c8d28b-5079-4ed8-a096-0412df687b5d", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2213092, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 36, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000036_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-36-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 36, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 37, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:01:04 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "9a3d0356-81f8-4470-a24b-1cd86b45b60a", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2214462, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 36, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000036_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-37-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 37, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 38, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:01:46 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "483b3349-7e56-4e98-9dc8-feb6c977fd75", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2256810, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 37, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000037_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-38-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 38, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 39, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:02:34 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "f1cc69c2-4653-4329-8834-c14cf8ca480e", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2304969, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 38, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000038_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-39-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 39, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 40, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:03:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "3ebb02fa-0e96-4319-a945-55a546740cab", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2354977, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 39, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000039_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-40-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 40, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 40, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:05:00 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "3e4aa3d0-f4b0-48be-9855-ad3bf952129c", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2450925, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 40, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000040_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-40-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 40, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 41, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:05:02 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "5964cc41-a037-451d-adbb-384b361058d8", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2452585, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 40, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000040_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-41-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 41, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 42, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:05:42 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "c6dcc05c-92b8-422f-8432-bac1051f067c", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2492723, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 41, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000041_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-42-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 42, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 43, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:06:33 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "733d4970-7955-46c5-8089-a9503ec5ed30", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2543862, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 42, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000042_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-43-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 43, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 44, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:07:25 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "436a7bb9-270d-409b-9f12-4752b70904f4", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2595990, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 43, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000043_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-44-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 44, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 44, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:08:59 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "51c1ab02-42cf-4eec-9030-b1dff59129f9", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2689177, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 44, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000044_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-44-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 44, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 45, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:09:00 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "6f1c80b0-f1bb-48e7-83b5-72350ca682a1", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2690798, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 44, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000044_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-45-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 45, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 46, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:09:43 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "d6a57cf0-3c34-4675-8bd8-e06a784f2c4b", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2733636, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 45, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000045_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-46-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 46, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 47, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:10:33 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "abec3a48-e56b-4817-a67b-31a483275023", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2783444, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 46, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000046_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-47-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 47, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 48, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:11:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "728c2e81-4314-4905-bfe0-d3f9207c1750", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2835053, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 47, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000047_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-48-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 48, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 48, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:12:59 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "b9303c0f-8b1a-4fc4-83f5-40e81054b5fe", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2929730, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 48, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000048_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-48-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 48, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 49, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:13:01 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "a288d71d-0449-4e8d-9348-c0223301de26", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2931161, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 48, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000048_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-49-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 49, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 50, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:13:46 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "d621a5a6-e02a-4b93-ac14-a02605682deb", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 2976340, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 49, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000049_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-50-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 50, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 51, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:14:33 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "ae60b6e4-dcc5-40ae-9723-828e4a1c7660", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3023648, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 50, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000050_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-51-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 51, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 52, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:15:24 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "c095298c-0012-4a5a-ac70-b4d2d0ecdf35", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3074976, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 51, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000051_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-52-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 52, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 52, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:16:58 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "50857b50-fb99-44f6-899c-f7ac1b0dd25a", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3169113, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 52, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000052_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-52-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 52, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 53, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:17:01 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "dd152dbd-141d-4d54-83b0-8583b0381b28", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3171154, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 52, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000052_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-53-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 53, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 54, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:17:42 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "0fc7b200-8f85-4594-9ec5-3883b3aebded", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3212592, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 53, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000053_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-54-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 54, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 55, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:18:33 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "b11409e1-70a1-4b8e-b276-8cebe22e2495", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3263870, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 54, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000054_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-55-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 55, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 56, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:19:25 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "fb08de58-f738-4843-a65c-81e3c9e6eefe", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3315309, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 55, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000055_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-56-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 56, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 56, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:21:01 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "10d35ba4-32d6-4168-8d6a-014ae5a0dd23", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3411816, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 56, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000056_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-56-1-0": { "Core-Focus": "TIPFSOffset", "Channel": "GFP", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 56, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 50, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "0", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 57, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:21:03 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 1, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "IntensiLightShutter", "TILightPath-State": "1", "UUID": "323cc654-7be8-4847-9d9f-2568149790cf", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3413176, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 56, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "50.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000056_GFP_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-57-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 57, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 58, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:21:48 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "78abeeeb-8521-4dd7-8948-e80c71e1c6ab", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3458895, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 57, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000057_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-58-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 58, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": 59, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:22:39 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "9c73425e-edbb-4525-b64e-0472e5d57f2a", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3509453, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 58, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000058_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" }, "FrameKey-59-0-0": { "Core-Focus": "TIPFSOffset", "Channel": "Phase[GFP]", "Core-Initialize": "1", "Arduino-Switch-Timed Output Mode": "Idle", "COM1-Verbose": "1", "Neo sCMOS-Description": "Andor SDK3 Device Adapter", "FrameIndex": 59, "XYStage-Version": "Version: USB-9.0h ", "Neo sCMOS-Sensitivity/DynamicRange": "11-bit (low noise)", "COM1-Description": "Serial port driver (boost:asio)", "TIFilterBlock1-Label": "2-EGFP", "Neo sCMOS-CameraName": "Andor sCMOS", "XYStage-Backlash-B": "0.0400", "Neo sCMOS-AccumulateCount": "1", "XYStage-Speed-S": "3.0000", "COM1-AnswerTimeout": "500.0000", "Camera": "", "Arduino-Shutter-Name": "Arduino-Shutter", "XYStage-Error-E(nm)": "400.0000", "XYStage-StepSizeX_um": "0.0100", "COM4-Parity": "None", "Core-ImageProcessor": "", "Arduino-Hub-Version": "2", "SlicePosition": null, "Neo sCMOS-TemperatureStatus": "Cooling", "Exposure-ms": 100, "XYStage-StepSizeY_um": "0.0100", "COM1-DataBits": "8", "TIPFSOffset-Position": "3.1250", "Core-AutoShutter": "1", "Arduino-Switch-State": "32", "TILightPath-Label": "Left100", "Arduino-Switch-Repeat Timed Pattern": "0", "XYStage-Name": "XYStage", "NextFrame": null, "TILightPath-Name": "", "Core-XYStage": "XYStage", "Neo sCMOS-AcquisitionWindow": "Full Image", "Neo sCMOS-Name": "Andor sCMOS Camera", "AxisPositions": null, "Time": "2013-09-24 19:23:27 -0700", "IntensiLightShutter-State": "0", "COM1-Parity": "None", "Arduino-Switch-Blank On": "Low", "COM4-Name": "COM4", "COM4-BaudRate": "57600", "XYStage-Port": "COM3", "TINosePiece-Label": "1- Plan Apo 100X Phase 1.45NA", "Core-AutoFocus": "TIPFSStatus", "COM1-StopBits": "1", "TIPFSOffset-Name": "TIPFSOffset", "BitDepth": 11, "TIFilterBlock1-State": "1", "Slice": 0, "TIPFSStatus-Name": "", "XYStage-JoyStick Slow Speed": "5", "COM4-Verbose": "0", "COM3-AnswerTimeout": "500.0000", "Neo sCMOS-PixelReadoutRate": "200 MHz - lowest noise", "Neo sCMOS-FanSpeed": "On", "Neo sCMOS-CameraID": "SCC-00889", "TINosePiece-Name": "TINosePiece", "TINosePiece-State": "0", "ChannelIndex": 0, "Arduino-Switch-Label": "0", "COM3-Handshaking": "Off", "Neo sCMOS-Binning": "1x1", "Core-Shutter": "Arduino-Shutter", "TILightPath-State": "1", "UUID": "c64355d0-3b7d-4d60-a244-57f53e22540a", "Core-Camera": "Neo sCMOS", "Core-TimeoutMs": "5000", "Core-SLM": "", "XYStage-JoyStick Fast Speed": "60", "Neo sCMOS-TransposeMirrorY": "0", "Neo sCMOS-TransposeMirrorX": "0", "ElapsedTime-ms": 3557892, "Arduino-Switch-Blanking Mode": "Idle", "COM1-Handshaking": "Off", "TIPFSStatus-State": "Off", "Neo sCMOS-SensorCooling": "On", "Neo sCMOS-TemperatureControl": "-30.00", "Arduino-Shutter-Description": "Arduino shutter driver", "IntensiLightShutter-Description": "Nikon IntensiLight Shutter adapter", "Frame": 59, "Core-ChannelGroup": "Channel", "PositionIndex": 15, "Width": 2560, "WaitInterval": null, "TIFilterBlock1-Name": "TIFilterBlock1", "IntensiLightShutter-Name": "IntensiLightShutter", "COM3-StopBits": "1", "PositionName": "Pos15", "PixelSizeUm": 0.0646, "Height": 2160, "IntensiLightShutter-ND": "8", "COM3-Verbose": "1", "Summary": { "Slices": 1, "Interval_ms": 60000, "UUID": "8ca1d044-14ee-49cf-93bf-4e8c34008d39", "UserName": "cellshape", "Depth": 2, "PixelType": "GRAY16", "Time": "2013-09-24 18:24:54 -0700", "Date": "2013-09-24", "z-step_um": 0, "MetadataVersion": 10, "PositionIndex": 15, "SlicesFirst": false, "ChContrastMin": [ 0, 0 ], "Width": 2560, "PixelAspect": 1, "MicroManagerVersion": "1.4.11 20121015", "ROI": [ 0, 0, 2560, 2160 ], "ChNames": [ "Phase[GFP]", "GFP" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2160, "GridColumn": 0, "Prefix": "BW25113_pAM_pSC_prcsF_phase_100ms_gfp_50ms", "PixelSize_um": 0.0646, "Frames": 60, "BitDepth": 11, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 2, "ComputerName": "kc-scope-02", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -1, -16711885 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536 ], "Positions": 16, "Directory": "S:\\Amanda\\09-24-13" }, "Source": "Neo sCMOS", "XYStage-OverShoot(um)": "0.0000", "Neo sCMOS-Overlap": "Off", "XYStage-Wait_Cycles": "0", "Arduino-Hub-Logic": "Normal", "COM4-StopBits": "1", "COM3-Name": "COM3", "Neo sCMOS-TriggerMode": "Internal (Recommended for fast acquisitions)", "IntensiLightShutter-Port": "COM1", "Neo sCMOS-Exposure": "100.00", "Arduino-Shutter-OnOff": "0", "COM4-DataBits": "8", "Arduino-Hub-Name": "Arduino-Hub", "XYStage-Maximum Speed, Do Not Change": "7.5000", "TIPFSStatus-Status": "Unknown", "COM3-DataBits": "8", "Arduino-Switch-Delay (ms)": "0", "XYStage-MotorOnOff": "On", "Neo sCMOS-TransposeXY": "0", "XYStage-Description": "ASI XY stage driver adapter", "Neo sCMOS-SpuriousNoiseFilter": "On", "Neo sCMOS-ElectronicShutteringMode": "Global", "Binning": "1x1", "COM4-Handshaking": "Off", "Arduino-Switch-HubID": "Arduino-Hub", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "COM1-DelayBetweenCharsMs": "0.0000", "COM4-DelayBetweenCharsMs": "0.0000", "Arduino-Shutter-HubID": "Arduino-Hub", "COM4-AnswerTimeout": "500.0000", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "Arduino-Switch-Name": "Arduino-Switch", "YPositionUm": 407.40000000000003, "COM3-Parity": "None", "COM1-BaudRate": "9600", "XYStage-TransposeMirrorY": "0", "XPositionUm": -19349, "XYStage-TransposeMirrorX": "0", "Neo sCMOS-SensorTemperature": "-26.0000", "Arduino-Hub-Port": "COM4", "IntensiLightShutter-Version": "V1.00", "Neo sCMOS-TransposeCorrection": "0", "FileName": "img_000000059_Phase[GFP]_000.tif", "Core-Galvo": "Undefined", "XYStage-JoyStick Reverse": "Off", "COM3-BaudRate": "9600", "COM1-Name": "COM1", "Arduino-Switch-Description": "Arduino digital output driver", "Neo sCMOS-PixelEncoding": "Mono12Packed", "CameraChannelIndex": 0, "SliceIndex": 0, "XYStage-FinishError-PCROS(nm)": "24.0000", "ZPositionUm": 27.55, "Arduino-Switch-Sequence": "Off" } }